dgk [2018-03-15 14:12:15]

deoxyguanosine kinase

locus: BSU00150

pI: 5.78

mw: 24.00 kDa

protein length: 207 aa

gene length: 621 bp

function: purine salvage and interconversion

product: deoxyguanosine kinase

essential: no

synonyms: yaaG

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.5|Nucleotide metabolism] → [category|SW 2.5.2|Biosynthesis/ acquisition of nucleotides] → [category|SW 2.5.2.3|Purine salvage and interconversion]

Gene

Coordinates: 23,146 → 23,769

The protein

Catalyzed reaction/ biological activity

• ATP + deoxyguanosine = ADP + dGMP. (according to SwissProt)

Structure

• [PDB|2JAQ] (DAK from Mycoplasma mycoides, 35% identity) [pubmed|17229440]

Expression and Regulation

Operons

• genes: [gene|0DA340B3D23B63C6BD284BF86409558A31B12B53|dgk]-[gene|D749382783437F40AE1C66755F57D44E3FCA193F|dck]
  description: [pubmed|22383849]

Biological materials

Mutant

• MGNA-B888 (yaaG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1887 NBRP B. subtilis, Japan]
• BKE00150 (Δ[gene|0DA340B3D23B63C6BD284BF86409558A31B12B53|dgk]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE00150 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCGGAATCCTCCTTATAT, downstream forward: _UP4_GCCCATGTAAAGGAGCTTAT
• BKK00150 (Δ[gene|0DA340B3D23B63C6BD284BF86409558A31B12B53|dgk]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK00150 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCGGAATCCTCCTTATAT, downstream forward: _UP4_GCCCATGTAAAGGAGCTTAT

References

• 11078735,17229440